Cytology Intro

Objectives:

 

I.          General considerations

            A.        Acquire a visual representation of the major morphologic features of the cell

                        (at both light microscopic and electron microscopic levels).

            B.         Correlate light microscopic images with ultrastructural features.

 

II.          The cell

            A.        Identify the following organelles:

                        1.         plasma membrane

                        2.         nucleus and nuclear membrane

                        3.         Golgi apparatus

                        4.         mitochondria

                        5.         endoplasmic reticulum (rough and smooth)

                        6.         lysosomes

 

            B.         Identify the following inclusions:

                        1.         glycogen

                        2.         lipid

                        3.         melanin

                        4.         protein secretion granules

 

            C.         Relate morphologic features of cells to physiologic function.

            1.         Examples include:

                                    a.         basophilic staining with nucleic acids and protein synthesis.

                                    b.         mitochondria with high energy requirement for ion transport.

                                    c.         uniform cytoplasmic particles with granules.

 

            D.         Review mitosis.

 

            E.         Identify the following stages of mitosis on tissue specimens:

                        1.         interphase

                        2.         prophase

                        3.         metaphase

                        4.         anaphase

                        5.         telophase

 

 

Slide List:

 

2.         Liver (also #166)

33.       Lymph node

3.         Epididymis

4.         Liver  - mitochondrial staining

32.       Uterus

6.         Pancreas

7.         Adrenal

8.         Liver

10.       Adipose Tissue

11.       Root tip

160.     Intestine